This study investigates learner autonomy from perspectives of non-majored English sophomores in a private university in the Mekong Delta. Both questionnaires and interviews were employed as the primary research instruments to analyze the collected data, utilizing a mixed-methods approach that combines qualitative and quantitative techniques. The findings of this study reveal factors affecting participants’ autonomy, including internal factors (learning motivation, learning attitudes, and learning strategies) and external factors (learning resources, learning environment, and the role of teacher).This study investigates learner autonomy from perspectives of non-majored English sophomores in a private university in the Mekong Delta. Both questionnaires and interviews were employed as the primary research instruments to analyze the collected data, utilizing a mixed-methods approach that combines qualitative and quantitative techniques. The findings of this study reveal factors affecting participants’ autonomy, including internal factors (learning motivation, learning attitudes, and learning strategies) and external factors (learning resources, learning environment, and the role of teacher).