Nghiên cứu hoạt tính sinh học của hoa đu đủ đực Carica papaya L. trên một số dòng tế bào ung thư phổ biến=Biological activity research of male papaya flowers Carica papaya L. on some common cancer cell lines

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Tác giả: Dung Ngô Thị Phương, Dũng Nguyễn Hoàng

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2024

Mô tả vật lý: tr.25-34

Bộ sưu tập: Báo, Tạp chí

ID: 252026

Thêm vào giỏ

Cây đu đủ đực Carica papaya L. được trồng phổ biến ở Việt Nam, hoa của nó có nhiều tác dụng dược lý. Nghiên cứu này đề cập đến các hoạt tính sinh học gồm hiệu quả kháng oxy hóa và chống ung thư của cao chiết phân đoạn hoa đu đủ đực. Hoa đu đủ đực được ly trích bằng dung môi methanol với hiệu suất tách chiết là 15.76%. Hiệu suất thu nhận cao chiết phân đoạn n-hexan, chloroform, ethyl acetate và nước lần lượt là: 11.48
7.32
8.05
73.15%. Hàm lượng polyphenol cao nhất trong cao phân đoạn nước là 88.15 ± 1.37 µg GAE/mg cao chiết, tương ứng với hiệu quả kháng oxy hóa tốt nhất với EC50 là 431.10 µg/mL được đo bằng phương pháp 1,1-diphenyl-2-picrylhydrazyl (DPPH). Dùng phương pháp 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) để kiểm tra hiệu quả gây độc tế bào, các dịch chiết phân đoạn đã ức chế tế bào ung thư HepG2, K562 và B16F10. Dịch chiết nước không có hoạt tính gây độc, nằm ngoài phạm vi khảo sát (IC50 >
400 µg/mL). Dịch chiết n-hexan ức chế tốt HepG2 (IC50 = 196.79 µg/mL), còn dịch chiết chloroform và ethyl acetate ức chế tốt K562 (IC50 = 83.23 µg/mL và 89.33 µg/mL). Nghiên cứu này được xem như bước đầu trong quá trình sàng lọc và cô lập các hợp chất sinh học từ hoa đu đủ đực ở tỉnh Đắk Lắk, tập trung vào các hợp chất có khả năng gây độc tế bào ung thư.The male papaya tree, Carica papaya L., is widely cultivated in Vietnam, and its flowers possess various pharmacological properties. This study focuses on the biological activities, including antioxidant and anticancer effects, of different fractions extracted from male papaya flowers. Male papaya flowers were extracted using methanol solvent with an extraction efficiency of 15.76%. The recovery efficiencies of the n-hexane, chloroform, ethyl acetate, and water fractions are 11.48, 7.32, 8.05, and 73.15%, respectively. The highest polyphenol content in the water fraction extract is 88.15 ± 1.37 µg GAE/mg extract, corresponding to the best antioxidant activity with an EC50 of 431.10 µg/mL measured by the DPPH method. Using the MTT method, the extract fractions also exhibited cytotoxic activity against HepG2 liver cancer cells, K562 leukemia cells, and B16F10 skin cancer cells. Except for the water extract, which falls outside the tested concentration range (IC50 >
400 µg/mL). The n-hexane fraction extract exhibited strong inhibition against HepG2 cells (IC50 = 196.79 µg/mL), while the chloroform and ethyl acetate fractions showed potent inhibition against K562 cells (IC50 = 83.23 µg/mL and 89.33 µg/mL, respectively). This study is considered an initial exploration in screening and isolating bioactive compounds from male papaya flowers in Dak Lak province, particularly those with cytotoxic activity against cancer cells

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