Fast event-based electron counting for small-molecule structure determination by MicroED.

• Tác giả: Duilio Cascio, Lygia Silva de Moraes, Hosea M Nelson, Songrong Qu, Logan S Richards, Jose A Rodriguez, Niko Vlahakis
• Ngôn ngữ: eng
• Ký hiệu phân loại: 242.4 Prayers and meditations for use in times of illness, trouble, bereavement
• Thông tin xuất bản: England : Acta crystallographica. Section C, Structural chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 252136

Tóm tắt

Electron counting helped realize the resolution revolution in single-particle cryoEM and is now accelerating the determination of MicroED structures. Its advantages are best demonstrated by new direct electron detectors capable of fast (kilohertz) event-based electron counting (EBEC). This strategy minimizes the inaccuracies introduced by coincidence loss (CL) and promises rapid determination of accurate structures. We used the Direct Electron Apollo camera to leverage EBEC technology for MicroED data collection. Given its ability to count single electrons, the Apollo collects high-quality MicroED data from organic small-molecule crystals illuminated with incident electron beam flux densities as low as 0.01-0.045 e