BACKGROUND: Eyebrows significantly influence facial aesthetics and are often linked to attractiveness and personality. Eyebrow loss and alopecia impact physical and psychological well-being. Several treatments, including mesotherapy (MT), aim to enhance eyebrow density and quality. Despite the availability of MT for hair growth, its application in eyebrow revitalization is less explored. AIMS: This study evaluates clients' perceptions and satisfaction with eyebrow MT (EB MT) using AQ skin solutions' growth factor-based MT cocktail serum. PATIENTS/METHODS: Thirty-two healthy women (ages 21-55) with thin or weakened eyebrows underwent three EB MT sessions spaced 2-4 weeks apart. Exclusion criteria included chronic diseases, allergies, and conditions like pregnancy. Each session lasted about 40 min, and "point by point" and "nappage" techniques were employed. Satisfaction and pain levels were assessed through a Numeric Rating Scale for Satisfaction (NRSS) and Pain scale, while photographic analysis and the Global Aesthetic Improvement Scale (GAIS) evaluated effectiveness. RESULTS: The average GAIS score was 8.28, while the NRSS satisfaction score was 8.06. Pain levels were moderately low, averaging 4.0. A high majority (90.6%) were satisfied and willing to continue treatment, with 100% recommending EB MT to others. Notable improvements in overall appearance were reported by participants. CONCLUSIONS: EB MT demonstrated high satisfaction and low pain levels, with significant improvements in eyebrow density and appearance. This treatment is a practical option for eyebrow enhancement with a strong safety profile, motivating further clinical application.