Phân lập, tuyển chọn và sơ bộ khảo sát đặc tính vi khuẩn chịu mặn có khả năng phân giải protein từ dịch chượp mắm truyền thống Nam Ô=Solation, screening, and preliminary characterization of salt-tolerant bacteria with the proteolytic ability from traditional nam-o fish sauce

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Tác giả: Thái Bích Vân Ngô, Khánh Đô Nguyễn, Trần Mỹ Hoàng Nguyễn, Châu Huỳnh Phạm, Thị Thu Hà Phan, Thị Thanh Thủy Trần, Văn Huy Hoàng Trương, Thuỳ Dương Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Đà Nẵng, 2024

Mô tả vật lý: tr.77-82

Bộ sưu tập: Báo, Tạp chí

ID: 252502

Thêm vào giỏ

Nghiên cứu này nhằm phân lập và tuyển chọn các dòng vi khuẩn chịu mặn có khả năng phân giải protein mạnh từ dịch chượp lên men nước mắm tại hai cơ sở sản xuất lớn của Làng nghề nước mắm truyền thống Nam Ô (thành phố Đà Nẵng). Qua việc phân lập trong điều kiện hiếu khí, đã thu được 79 dòng vi khuẩn. Bằng phương pháp cấy vạch và khuếch tán trên đĩa thạch, 11 dòng được xác định có khả năng sinh protease mạnh, với kích thước vòng phân giải sữa gầy >
15mm. Cả 11 dòng này cho thấy, khả năng tăng trưởng và sản sinh protease tốt trong môi trường lỏng chứa 4,0w% NaCl, và 3 trong số chúng giữ được khả năng này trong môi trường có 20,0w% NaCl. Kết quả khảo sát các yếu tố ảnh hưởng đến sự phát triển cho thấy, điều kiện thích hợp để thu sinh khối của các dòng này là nhiệt độ 37°C, và thời gian 16-20 giờ. Kết quả giải trình tự gene 16S rRNA cho phép định danh 3 dòng vi khuẩn này thuộc chi Bacillus .This study aimed to isolate and select salt-tolerant bacterial strains with strong proteolytic ability from fish sauce fermentation broth at two major manufacturers in the Nam-O Traditional Fish Sauce Village (Danang City). By isolation under aerobic conditions, 79 bacterial strains were obtained. Using the streak culture and agar plate diffusion methods, 11 strains were identified as having strong proteolytic activity with a clear zone diameter of >
15mm. All 11 strains showed good growth and protease production in liquid media containing 4.0w% NaCl, and three of them retained this ability in media with 20.0w% NaCl. The survey of growth factors showed that,the appropriate conditions for biomass production of these strains were a temperature of 37°C and a culture period of 16-20 hours. Sequencing of the 16S rRNA gene allowed the identification of three bacterial strains belonging to the Bacillusgenus.

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