Biểu hiện giảm của lncRNA UCA1 trong exosome huyết tương của bệnh nhân ung thư phổi không tế bào nhỏ=Downregulated expression of exosomal long non-coding RNA UCA1 in plasma of patients with non-small cell lung cancer

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Tác giả: Thị Tú Linh Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học và Công nghệ Việt Nam, 2024

Mô tả vật lý: tr.21-27

Bộ sưu tập: Báo, Tạp chí

ID: 252521

Thêm vào giỏ

Các phân tử RNA dài không ghi mã (lncRNA) có chủ yếu trong nhân của tế bào và tham gia vào nhiều quá trình điều hoà biểu hiện gen khác nhau. Mức độ biểu hiện bất thường của các phân tử lncRNA được cho là có vai trò đối với hoạt động di căn, xâm lấn và tăng sinh của khối u, trong đó có ung thư phổi không tế bào nhỏ (UTPKTBN). Các lncRNA cũng được phát hiện thấy có biểu hiện tăng hoặc giảm trong các exosome do tế bào ung thư tiết ra, do đó chúng có tiềm năng được sử dụng như một chỉ thị sinh học ung thư không xâm lấn. Trong nghiên cứu này, mức độ biểu hiện của lncRNA UCA1 trong exosome huyết tương được xác định trên 29 mẫu bệnh nhân được chẩn đoán mắc UTPKTBN và 29 mẫu huyết tương đối chứng được lấy từ người không mắc bệnh UTPKTBN và các bệnh liên quan đến phổi bằng phương pháp PCR định lượng (qPCR) sử dụng SYBR Green. Sau đó, các phương pháp phân tích thống kê được sử dụng để đánh giá mối liên quan giữa mức độ biểu hiện của lncRNA UCA1 với các đặc điểm bệnh học của UTPKTBN. Kết quả cho thấy, lncRNA UCA1 có biểu hiện giảm trong exosome huyết tương của bệnh nhân UTPKTBN so với nhóm đối chứng (p=0,05), đặc biệt là giảm ở giai đoạn sớm (giai đoạn I + II). Tuy nhiên, biểu hiện của lncRNA UCA1 không có mối liên quan với các đặc điểm bệnh học của UTPKTBN như độ tuổi, giới tính, tình trạng hút thuốc lá và uống rượu, kích thước khối u, giai đoạn bệnh, mức độ xâm lấn, mức độ hạch và mức độ di căn của khối u. Nghiên cứu đã cung cấp các số liệu ban đầu về biểu hiện của lncRNA UCA1 trong exosome huyết tương của bệnh nhân UTPKTBN người Việt Nam làm tiền đề cho các nghiên cứu sâu hơn về lncRNA trên nhóm bệnh nhân này.Long non-coding RNAs (lncRNAs), mainly found in the nucleus of cells, are involved in different gene expression and regulation processes. Growing evidence has shown that abnormalities in the expression levels of lncRNAs play an important role in tumour metastasis, invasion, and proliferation, especially in non-small cell lung cancer (NSCLC). LncRNAs were also found to be upregulated or downregulated in exosomes secreted by cancer cells, thus they have the potential to be used as a non-invasive cancer biomarker. In this study, the expression of exosomal lncRNA UCA1 was determined on 29 plasma samples of patients with NSCLC and 29 controls who did not have NSCLC or related lung diseases by quantitative PCR using SYBR Green. Then, statistical analyses were used to evaluate the association between the expression of lncRNA UCA1 and some pathological features of NSCLC. The results showed that lncRNA UCA1 was downregulated in the exosomes of NSCLC patients compared with the control group (p=0.050), especially in the early stage (stage I + II). However, there was no association between the expression of lncRNA UCA1 and some pathological features of NSCLC, such as age, sex, smoking, alcohol consumption, tumour size, disease stage, lymph nodes, or tumour metastasis. This study provided the initial data on the expression of exosomal lncRNA UCA1 in the plasma of Vietnamese patients with NSCLC and further studies on the larger samples are needed to be continued.

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