Phân tích đa hình vùng exon 1 gen OsHKT1;5 trên các giống lúa mùa chịu mặn tỉnh Kiên Giang=Polymorphic analysis of the exon 1 region of oshkt1;5 gene in Kien Giang seasonal rice salt tolerance

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Tác giả: Thi Pha Nguyễn, Hoàng Thanh Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Nông nghiệp và Phát triển Nông thôn, 2024

Mô tả vật lý: tr.11-20

Bộ sưu tập: Metadata

ID: 252801

Thêm vào giỏ

Nguy cơ xâm nhập mặn do biến đổi khí hậu ngày càng nghiêm trọng, đe dọa sản xuất lúa ở vùng đồng bằng sông Cửu Long (ĐBSCL). Nghiên cứu đánh giá tính chống chịu mặn của 16 giống lúa mùa địa phương thu thập từ tỉnh Kiên Giang so sánh với Pokkali và RC222 đã chọn ra được các giống lúa có khả năng chống chịu mặn khác nhau. Các giống lúa này sau đó được phân tích đa hình vùng exon 1 gen Os HKT1
5 bằng kỹ thuật giải trình tự. Kết quả đánh giá kiểu hình đã xác định được 7 giống lúa có tính chống chịu mặn khác nhau gồm: Lúa Chuối, FL478, Pokkali có khả năng chống chịu tốt
Nàng Trích, Trắng Tép, Ba Bụi, Một Bụi có tính chống chịu mặn trung gian ở các mức khác nhau
RC222 mẫn cảm với mặn. Phân tích trình tự vùng exon 1 gen Os HKT1
5 đã xác định được 3 dấu SNP ở các vị trí nucleotide G382A, C418G và G551A. Cả 3 dấu SNP đều là đột biến sai nghĩa làm thay đổi các amino axit D128N, P140A và R184H. Các SNP G382A và G551A có thể có liên quan đến tính chống chịu mặn của các giống lúa.The increasing risk of saltwater intrusion due to climate change is a serious threat to rice production in the Mekong Delta. In order to address this issue, a study was conducted to evaluate the salt tolerance of 16 local seasonal rice varieties collected from Kien Giang province. These varieties were compared to the well-known salt-tolerant varieties Pokkali and RC222. The aim was to identify rice varieties with varying levels of salt tolerance. In addition, the study also analyzed the exon 1 region of the OsHKT1
5 gene in these varieties using sequencing techniques. The results of the phenotypic evaluation revealed that 7 of the rice varieties showed different levels of salt tolerance. These included Lua Chuoi, FL478, and Pokkali (which showed good tolerance), as well as Nang Trich, Trang Tep, Ba Bui, and Mot Bui (which showed intermediate salt tolerance). However, RC222 was found to be sensitive to salinity. Further analysis of the exon 1 region of the OsHKT1
5 gene identified 3 SNP markers at G382A, C418G, and G551A nucleotide positions. These markers were found to be non-synonymous mutations, resulting in changes in the amino acids D128N, P140A, and R184H, respectively.

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Phân tích đa hình vùng exon 1 gen O­sHKT1;5 trên các giống lúa mùa chịu mặn tỉnh Kiên Giang=Polymorphic analysis of the exon 1 region of oshkt1;5 gene in Kien Giang seasonal rice salt tolerance

Phân tích đa hình vùng exon 1 gen OsHKT1;5 trên các giống lúa mùa chịu mặn tỉnh Kiên Giang=Polymorphic analysis of the exon 1 region of oshkt1;5 gene in Kien Giang seasonal rice salt tolerance