Root canal obturation involves filling of the chemomechanically prepared root canal space. Despite reduced microbial load, residual bacteria can still lead to reinfection and treatment failure. Currently, obturation techniques use a combination of gutta-percha and sealer, which requires the preparation of the root canal to specific sizes and tapers to enable the fitting of customized cones. This study aims to characterize the physical, chemical, and antimicrobial properties of a new light-curable injectable material (OdneFill, Switzerland) used to obturate the root canal. Odnefill and 2 root canal sealers (AH Plus and BioRoot RCS) were characterized by scanning electron microscopy (SEM) and energy-dispersive spectroscopy following exposure to chlorhexidine, sodium hypochlorite, and water. The flow, film thickness, radiopacity, solubility, and contact angle were evaluated. The susceptibility to microbial degradation was assessed by weight changes after contact with bacterial enzymes (lipase and cholesterol esterase). A multispecies biofilm composed of