Tối ưu hoá điều kiện nuôi cấy các chủng vi sinh vật hữu ích để tạo chế phẩm xử lý chất thải rắn hữu cơ thành phân bón=Optimization of culture condition for useful microbial strains to treat organic solid waste into fertilizers

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Tác giả: Thị Bích Liễu Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Nông nghiệp và Phát triển Nông thôn, 2024

Mô tả vật lý: tr.54-65

Bộ sưu tập: Metadata

ID: 252815

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Nghiên cứu này đã tuyển chọn và tối ưu hóa môi trường lên men thu sinh khối ba chủng vi sinh hữu ích: Bacillus velezensis N 24 , Bacillus amyloliquefaciens NT và Trichoderma sp. N 1 với đặc tính các chủng có khả năng chuyển hóa các hợp chất hữu cơ đa dạng (tinh bột, cellulose, protein và pectin), có khả năng sinh tổng hợp IAA, phân giải phốt phát khó tan và cố định nitơ, đối kháng vi sinh vật gây bệnh. Các thông số tối ưu môi trường lên men thu sinh khối các chủng bao gồm: Chủng N 24 bổ sung 4,5 g/l tinh bột, 8,25 g/l pepton, pH = 7, nhiệt độ 34 o C
tốc độ lắc 150 vòng/phút, hàm lượng sinh khối ướt chủng N 24 đạt 27,55 g/l. Chủng NT lên men trong môi trường chứa 3,87 g/l tinh bột, 8 g/l pepton, pH = 7, nhiệt độ 35 o C
tốc độ lắc 150 vòng/ phút, hàm lượng sinh khối ướt đạt 29,32 g/l
trong môi trường chứa 5g/l tinh bột
6,85 g/l pepton
pH = 6
nhiệt độ 32 o C
tốc độ lắc 77 vòng/phút, hàm lượng sinh khối ướt chủng N 1 đạt 39,21 g/l. Bước đầu đã tạo được chế phẩm vi sinh vật trên chất mang để xử lý chất thải hữu cơ thành phân bón đạt tiêu chuẩn.This study selected and optimized the fermentation medium for biomass collection of three useful microbial strains: Bacillus velezensis N24, Bacillus amyloliquefaciens NT and Trichoderma sp. N1 with the characteristics of strains capable of metabolizing diverse organic compounds (starch, cellulose, protein, and pectin), as well as capable of biosynthesizing IAA, degrading insoluble phosphate, and fixing nitrogen, resistance to pathogenic microorganisms. The recommended process parameters for N24 strain biomass collection of strains are medium supplemented with 4.5 g/l starch, 8.25 g/l peptone, innitial pH 7, temperature 34oC, 150 rpm shaking speed. Under these conditions, the wet biomass content of strain N24 can be reached 27.55 g/l. For the NT strain, fermented medium supplemented with 3.87 g/l starch, 8g/l peptone, innitial pH 7, temperature 35oC, shaking speed 150 rpm, the wet biomass content reached 29.32 g/l. For the N1 strain, fermented medium containing 5 g/l starch
6.85 g/l peptone
innitial pH 6
temperature 32oC, shaking speed 77 rpm, the wet biomass content reached 39.21 g/l. Initially, a microbial product was created on the carrier to process organic waste into standard fertilizer.

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