Nuôi cấy quả thể Đông trùng hạ thảo (Cordyceps militaris) trên cơ chất rắn bổ sung vi tảo=Culturing fruit bodies of Cordyceps militaris on solid substrate supplemented with microalgae

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Minh Tâm Lê, Thị Bảo Trâm Trần, Thị Tư Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học và Công nghệ Lâm nghiệp, 2024

Mô tả vật lý: tr.20-28

Bộ sưu tập: Metadata

ID: 253123

Thêm vào giỏ

Cordyceps militaris is a valuable medicinal mushroom with high economic value, but it is overexploited in nature, leading to scarcity. Many studies have been conducted on growing C. militaris fruiting bodies on solid substrates supplemented with various nutrients. Microalgae (chlorella and spirulina) contain large amounts of protein (more than 50% of dry weight) and vitamins suitable for the growth of C. militaris fruiting bodies. In this study, C. militaris was cultivated under appropriate conditions and the concentration of microalgae added to the solid substrate for cultivating C. militaris fruiting bodies (chlorella: 5%, 10%, 15%, 20% – spirulina: 5%, 10%, 15%, 20%) Analysis of cordycepin and adenosine content was performed with the control sample, the best treatment of green algae and spirulina. The results showed that C. militaris grew on all treatments examined, in which the therapy supplemented with 20% chlorella (height: 63.98 ± 1.78 mm, diameter: 2.43 ± 0.20 mm, quantity of fruiting bodies: 132.5 ± 17.00 fibers, mass: 91.61 ± 1.90 g, cordycepin content: 3975 mg/kg, adenosine content: 201 mg/kg) and 15% spirulina (height: 76.23 ± 1.17 mm, diameter: 2.77 ± 0.33 mm, quantity of fruiting bodies: 153.8 ± 10.40 fibers, mass: 92.24 ± 2.43 g, cordycepin content: 4015 mg/kg, adenosine content: 244 mg/kg) gave the best yield and quality. The solid culture medium supplemented with spirulina produced C. militaris fruiting bodies with better yield and quality than chlorella and the control. This technique can be applied to deliver and improve the yield and quality of C. militaris fruiting bodies.Đông trùng hạ thảo – Cordyceps militaris là nấm dược liệu quý, có giá trị kinh tế cao. Đã có nhiều nghiên cứu nuôi cấy C. militaris trên cơ chất rắn bổ sung nhiều chất dinh dưỡng khác nhau. Vi tảo (tảo lục và tảo xoắn) chứa lượng protein cao và các loại vitamin phù hợp cho quá trình nuôi cấy quả thể C. militaris. Trong nghiên cứu này, C. militaris được nuôi cấy ở điều kiện thích hợp và khảo sát nồng độ vi tảo bổ sung vào cơ chất rắn nuôi cấy quả thể (tảo lục: 5%, 10%, 15%, 20% - tảo xoắn: 5%, 10%, 15%, 20%). Phân tích hàm lượng cordycepin và adenosine đối với nghiệm thức tốt nhất của tảo lục và tảo xoắn. Kết quả cho thấy C. militaris sinh trưởng, phát triển trên tất cả các nghiệm thức được khảo sát, trong đó nghiệm thức bổ sung 20% tảo lục (chiều cao quả thể: 63,98 ± 1,78 mm, đường kính quả thể: 2,43 ± 0,20 mm, số lượng quả thể: 132,5 ± 17,00, khối lượng quả thể 91,61 ± 1,90 g/hộp, hàm lượng Cordycepin: 3975 mg/kg, hàm lượng adenosine: 201 mg/kg) và 15% tảo xoắn (chiều cao quả thể : 76,23 ± 1,17 mm, đường kính quả thể: 2,77 ± 0,33 mm, số lượng quả thể: 153,8 ± 10,40 sợi, khối lượng quả thể: 92,24 ± 2,43 g/hộp, hàm lượng Cordycepin: 4015 mg/kg, hàm lượng adenosine: 244 mg/kg) cho năng suất và chất lượng tốt nhất. Môi trường nuôi cấy rắn bổ sung tảo xoắn thu được quả thể có năng suất và chất lượng tốt hơn so với tảo lục và đối chứng. Kỹ thuật này có thể áp dụng sản xuất và nâng cao sản lượng và chất lượng quả thể C. militaris.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR