Structural characterization of pyruvic oxime dioxygenase, a key enzyme in heterotrophic nitrification.

• Tác giả: Taketomo Fujiwara, Akihiko Nakamura, Miki Senda, Toshiya Senda, Shuhei Tsujino, Yusuke Yamada
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : Journal of bacteriology , 2025
• Bộ sưu tập: NCBI
• ID: 253544

Tóm tắt

Nitrification by heterotrophic microorganisms is an important part of the nitrogen cycle in the environment. The enzyme responsible for the core function of heterotrophic nitrification is pyruvic oxime dioxygenase (POD). POD is a non-heme, Fe(II)-dependent enzyme that catalyzes the dioxygenation of pyruvic oxime to produce pyruvate and nitrite. To analyze the catalytic mechanism of POD, the crystal structure of POD from