In this work, chitosan (Cs) was blended with different concentrations of Origanum majorana extract (OmE) that extracted using ethyl acetate and used for the formation of food packaging films. Based on the utilized volumes of OmE (2.5, 5, and 7.5 mL) that were mixed with 27.5, 25, and 22.5 mL of Cs, these different film samples (2.5 OmE loaded Cs film, 5 OmE loaded Cs film and 7.5 OmE loaded Cs film) were obtained and compared with the film of pure Cs. The extraction yields of OmE were found to be 13 g and 10.4 g % when using ethyl acetate (OmE) and methanol (OmME), respectively, with total phenolic content measured at 889.30 μg GAE/g for the ethyl acetate extract and 810.21 μg GAE/g for the methanol extract, indicating a substantial amount of bioactive compounds available for formulation. Antimicrobial activity was assessed against various foodborne pathogens, with the 7.5 OmE loaded Cs film demonstrating the highest efficacy, achieving inhibition zones of 27 mm against E. coli and 25 mm against S. aureus. This research underscores the potential of Cs-based films loaded with O. majorana extract as a viable solution for active food packaging, addressing environmental concerns and food safety.