The study aims to evaluate how bacteriocin and extracellular polymeric substances (EPS) can influence the development of active packaging for food. The components might enhance the performance of packaging materials in terms of their physicochemical properties and their effectiveness in preserving food. Bacteriocin and EPS exert a significant effect in blocking the transmission of UV and visible light radiations. The molecular stability among the different functional groups of the composite films was evaluated using FT-IR analysis. The MG5 film exhibited the lowest percentage of water solubility (11.27 %) and the highest antibacterial activity against L. monocytogenes and E. coli, with a zone of inhibition measured as 21.32 ± 0.76 and 18.81 ± 0.29 mm, respectively. The TGA results indicated a noteworthy level of thermal stability in the composite films. Specifically, the MG5 bacteriocin blended film exhibited an approved metal chelation activity and demonstrated superior antioxidant activity, as evidenced by enhanced DPPH and ABTS