WD40 superfamily genes are integral to various aspects of plant growth and development. Despite the economic importance and agricultural significance of the kiwifruit (Actinidia chinensis), a comprehensive characterization of the WD40 superfamily in this species remains elusive. In this study, we identified 280 WD40-encoding genes within the kiwifruit genome and systematically analyzed their phylogenetic relationships, gene structures, functional domains, and synteny. Our results reveal that AcWD40 genes exhibit diverse expression profiles with distinct spatio-temporal patterns. AcWD40.063, encoding TTG1 homolog (designated AcTTG1), was upregulated during light-induced anthocyanin accumulation. Heterologous expression, yeast two-hybrid (Y2H) interaction assays, and dual-luciferase reporter experiments revealed that AcTTG1 interacts with AcMYB75 and AcbHLH2, collectively promoting anthocyanin accumulation and enhancing the expression of anthocyanin biosynthesis genes, particularly AcANS. This study provides a robust framework for understanding the roles of AcWD40 gene family members and offers valuable insights for molecular breeding strategies aimed at improving kiwifruit quality.