Anti-inflammatory activity, stability, bioavailability and toxicity studies on seabuckthorn polyphenol enriched fraction and its phospholipid complex (Phytosomes) preparation.

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Tác giả: Arvind Kumar Bansal, Dattatraya Dinkar Gore, Sanjay Madhukar Jachak, Gopabandhu Jena, Dinesh Kumar, Nidhi Mishra, Inder Pal Singh, Kulbhushan Tikoo

Ngôn ngữ: eng

Ký hiệu phân loại: 942.0164 *England and Wales

Thông tin xuất bản: Netherlands : International journal of biological macromolecules , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 254139

A standardized polyphenol-enriched fraction (IPHRFPPEF) was formulated into a phospholipid complex (IPHRFPPEF-PC) to enhance oral bioavailability and evaluate stability, toxicity, and in vivo anti-inflammatory activity in Sprague Dawley rats. IPHRFPPEF was prepared from crude extract using XAD-HP7/Diaion-HP20 resin column chromatography and analyzed via HPLC and NMR. Total phenolic and flavonoid contents were quantified, with IPHRFPPEF showing higher values than the crude fraction. The phospholipid complex was prepared via solvent evaporation and assessed for bioavailability, stability, and toxicity. Key results demonstrated a 1.99-fold, 2.03-fold, and 1.66-fold increase in plasma concentrations of isorhamnetin, kaempferol and quercetin respectively. Acute oral toxicity testing showed an LD
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