Engineered nanomedicine for neuroregeneration [electronic resource] : light emitting diode-mediated superparamagnetic iron oxide-gold core-shell nanoparticles functionalized by nerve growth factor

• Ngôn ngữ: eng
• Ký hiệu phân loại: 541.3 Miscellaneous topics in physical chemistry
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2019
• Mô tả vật lý: Size: Article No. 102052 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 259983
• Chủ đề: 1. 60 applied life sciences; 2. Calcium influx; 3. Light emitting diode; 4. Nerve regeneration; 5. Ngf-spio-au nanoparticles; 6. Photothermal effect; 7. Research & experimental medicine; 8. Science & technology

Tóm tắt

 This article reports nerve growth factor functionalized superparamagnetic iron oxide?gold core-shell nanoparticles (NGF-SPIO-Au NPs), an engineered nanomedicine for non-invasive neuron regeneration when irradiated by a low-intensity light-emitting diode (LED). NGF-SPIO-Au NPs of 20 ?g/ml, were tested on PC-12 neuron-like cells, irradiated by LEDs (525 nm, 1.09, 1.44, and 1.90 mW/cm2). A remarkable Ca<
 sup>
 2+<
 /sup>
  influx was detected in differentiated PC-12 cells treated with NPs, irradiated by LED of 1.90 and 1.44 mW/cm<
 sup>
 2<
 /sup>
  with great cell viability (>
 84%) and proliferations. The strong heat generated through their plasmonic surface upon LED irradiation on NGF-SPIO-Au NPs was observed. For cells treated with LED (1.90 mW/cm<
 sup>
 2<
 /sup>
 ) and NGF-SPIO-Au NPs, a dramatic enhancement of neuronal differentiation (83%) and neurite outgrowth (51%) was found, and the upregulation of both the neural differentiation specific marker (?3-tubulin) and the cell adhesive molecule (integrin ?1) was observed by the reverse transcription-polymerase chain reaction and western blot analysis.