A metabolic pathway for catabolizing levulinic acid in bacteria [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 576.8 Evolution

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017

Mô tả vật lý: Size: p. 1624?1634 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 260356

 Microorganisms can catabolize a wide range of organic compounds and therefore have the potential to perform many industrially relevant bioconversions. One barrier to realizing the potential of biorefining strategies lies in our incomplete knowledge of metabolic pathways, including those that can be used to assimilate naturally abundant or easily generated feedstocks. For instance, levulinic acid (LA) is a carbon source that is readily obtainable as a dehydration product of lignocellulosic biomass and can serve as the sole carbon source for some bacteria. Yet, the genetics and structure of LA catabolism have remained unknown. Here, we report the identification and characterization of a seven-gene operon that enables LA catabolism in <
 i>
 Pseudomonas putida<
 /i>
  KT2440. When the pathway was reconstituted with purified proteins, we observed the formation of four acyl-CoA intermediates, including a unique 4-phosphovaleryl-CoA and the previously observed 3-hydroxyvaleryl-CoA product. Using adaptive evolution, we obtained a mutant of <
 i>
 Escherichia coli<
 /i>
  LS5218 with functional deletions of <
 i>
 fadE<
 /i>
  and <
 i>
 atoC<
 /i>
  that was capable of robust growth on LA when it expressed the five enzymes from the <
 i>
 P. putida<
 /i>
  operon. Here, this discovery will enable more efficient use of biomass hydrolysates and metabolic engineering to develop bioconversions using LA as a feedstock.
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