Advances in microscale separations towards nanoproteomics applications [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 570.15 Scientific principles
• Thông tin xuất bản: Richland, Wash. : Oak Ridge, Tenn. : Pacific Northwest National Laboratory (U.S.) ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: p. 40-48 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260408
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Capillary electrophoresis; 4. Mass spectrometry; 5. Microscale separations; 6. Nanolc; 7. Nanoproteomics

Tóm tắt

Microscale separation (e.g., liquid chromatography or capillary electrophoresis) coupled with mass spectrometry (MS) has become the primary tool for advanced proteomics, an indispensable technology for gaining understanding of complex biological processes. In recent decades significant advances have been achieved in MS-based proteomics. But, the current proteomics platforms still face an analytical challenge in overall sensitivity towards nanoproteomics applications for starting materials of less than 1 ?g total proteins (e.g., cellular heterogeneity in tissue pathologies). We review recent advances in microscale separation techniques and integrated sample processing strategies that improve the overall sensitivity and proteome coverage of the proteomics workflow, and their contributions towards nanoproteomics applications.