Instructing cells with programmable peptide DNA hybrids [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 546 Inorganic chemistry
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: Article No. 15982 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260437
• Chủ đề: 1. 36 materials science; 2. 59 basic biological sciences; 3. 60 applied life sciences; 4. 99 general and miscellaneous; 5. Bioinspired materials; 6. Bioinspired materials biomaterials ? cells biomedical materials; 7. Biomaterials - cells; 8. Biomedical materials

Tóm tắt

The native extracellular matrix is a space in which signals can be displayed dynamically and reversibly, positioned with nanoscale precision, and combined synergistically to control cell function. Here we describe a molecular system that can be programmed to control these three characteristics. In this approach we immobilize peptide-DNA (P-DNA) molecules on a surface through complementary DNA tethers directing cells to adhere and spread reversibly over multiple cycles. The DNA can also serve as a molecular ruler to control the distance-dependent synergy between two peptides. Finally, we use two orthogonal DNA handles to regulate two different bioactive signals, with the ability to independently up- or downregulate each over time. This enabled us to discover that neural stem cells, derived from the murine spinal cord and organized as neurospheres, can be triggered to migrate out in response to an exogenous signal but then regroup into a neurosphere as the signal is removed.