Identification and characterization of a class of MALAT1 -like genomic loci [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 570 Life sciences Biology
• Thông tin xuất bản: Richland, Wash. : Oak Ridge, Tenn. : Pacific Northwest National Laboratory (U.S.) ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: p. 1723-1738 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260473
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Et al; 4. Evolution; 5. Long noncoding rna; 6. Malat1; 7. Pirna; 8. Piwi; 9. Testis; 10. Triple helix; 11. Trna-like

Tóm tắt

 The <
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 MALAT1<
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  (Metastasis-Associated Lung Adenocarcinoma Transcript 1) gene encodes a noncoding RNA that is processed into a long nuclear retained transcript (<
 i>
 MALAT1<
 /i>
 ) and a small cytoplasmic tRNA-like transcript (mascRNA). Using an RNA sequence- and structure-based covariance model, we identified more than 130 genomic loci in vertebrate genomes containing the <
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 MALAT1<
 /i>
  3' end triple-helix structure and its immediate downstream tRNA-like structure, including 44 in the green lizard <
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 Anolis carolinensis<
 /i>
 . Structural and computational analyses revealed a co-occurrence of components of the 3' end module. <
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 MALAT1<
 /i>
 -like genes in <
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 Anolis carolinensis<
 /i>
  are highly expressed in adult testis, thus we named them testis-abundant long noncoding RNAs (tancRNAs). <
 i>
 MALAT1<
 /i>
 -like loci also produce multiple small RNA species, including PIWI-interacting RNAs (piRNAs), from the antisense strand. The 3' ends of tancRNAs serve as potential targets for the PIWI-piRNA complex. Furthermore, we have identified an evolutionarily conserved class of long noncoding RNAs (lncRNAs) with similar structural constraints, post-transcriptional processing, and subcellular localization and a distinct function in spermatocytes.