Quantifying Mesoscale Neuroanatomy Using X-Ray Microtomography [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 620.1 Engineering mechanics and materials

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017

Mô tả vật lý: Size: Article No. ENEURO.0195-17.2017 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 260536

 Methods for resolving the three-dimensional (3D) microstructure of the brain typically start by thinly slicing and staining the brain, followed by imaging numerous individual sections with visible light photons or electrons. In contrast, X-rays can be used to image thick samples, providing a rapid approach for producing large 3D brain maps without sectioning. Here we demonstrate the use of synchrotron X-ray microtomography (�CT) for producing mesoscale (~1 �m <
 sup>
 3<
 /sup>
  resolution) brain maps from millimeter-scale volumes of mouse brain. We introduce a pipeline for �CT-based brain mapping that develops and integrates methods for sample preparation, imaging, and automated segmentation of cells, blood vessels, and myelinated axons, in addition to statistical analyses of these brain structures. Our results demonstrate that X-ray tomography achieves rapid quantification of large brain volumes, complementing other brain mapping and connectomics efforts.
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