Endoribonuclease-Based Two-Component Repressor Systems for Tight Gene Expression Control in Plants [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 580.7 Education, research, related topics
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: p. 806-816 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260648
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Crisprcas9; 4. Csy4; 5. Endoribonuclease; 6. Gene regulation; 7. Synthetic biology; 8. Two-component repressor system

Tóm tắt

 Tight control and multifactorial regulation of gene expression are important challenges in genetic engineering and are critical for the development of regulatory circuits. In meeting these challenges we will facilitate transgene expression regulation and support the fine-tuning of metabolic pathways to avoid the accumulation of undesired intermediates. By employing the endoribonuclease Csy4 and its recognition sequence from Pseudomonas aeruginosa and manipulating 5'UTR of mRNA, we developed a two-component expression?repression system to tightly control synthesis of transgene products. We demonstrated that this regulatory device was functional in monocotyledonous and dicotyledonous plant species, and showed that it can be used to repress transgene expression by >
 400-fold and to synchronize transgene repression. In addition to tissue-specific transgene repression, this system offers stimuli-dependent expression control. Here, we identified 54 orthologous systems from various bacteria, using a bioinformatics approach and then validated in planta the activity for a few of those systems, demonstrating the potential diversity of such a two-component repressor system.