Cytosolic delivery of materials with endosome-disrupting colloids [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 630.6 Agriculture and related technologies
• Thông tin xuất bản: Berkeley, Calif. : Oak Ridge, Tenn. : Lawrence Berkeley National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016
• Mô tả vật lý: Medium: ED : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260917
• Chủ đề: 1. 47 other instrumentation; 2. 60 applied life sciences; 3. 77 nanoscience and nanotechnology

Tóm tắt

A facile procedure to deliver nanocrystals to the cytosol of live cells that is both rapid and general. The technique employs a unique cationic core-shell polymer colloid that directs nanocrystals to the cytosol of living cells within a few hours of incubation. The present methods and compositions enable a host of advanced applications arising from efficient cytosolic delivery of nanocrystal imaging probes: from single particle tracking experiments to monitoring protein-protein interactions in live cells for extended periods.