Tunable, Ultrafast Fiber-Laser between 1.15 ?m and 1.35 ?m for Harmonic Generation Microscopy in Human Skin [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 545 Quantitative analysis
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2018
• Mô tả vật lý: Size: Article No. 6800708 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261064
• Chủ đề: 1. 60 applied life sciences; 2. Biomedical imaging; 3. Fiber lasers; 4. Nonlinear fiber optics; 5. Nonlinear optical microscopy; 6. Optical harmonic generation

Tóm tắt

 We demonstrate a fiber-optic ultrafast tunable source for harmonic generation microscopy (HGM) in human skin. The source is based on a 31-MHz Er-fiber laser followed by self-phase modulation enabled spectral selection (SESS). The resulting pulses are tunable between 1.15 and 1.35 ?m with up to >
 10-nJ pulse energy and ~100-fs pulse duration. Here we employ this source to drive a scanning microscope for HGM imaging of ex vivo human skin. A systematic investigation on imaging depth versus excitation wavelength reveals that excitation wavelengths in the 1.15-1.25 ?m range exhibit low optical attenuation within the tissue and allows larger imaging depth for HGM in human skin. HGM driven by fiber-based SESS sources constitutes an enabling tool for noninvasive virtual skin biopsy in clinical applications.