Identification of bacteria synthesizing ribosomal RNA in response to uranium addition during biostimulation at the Rifle, CO Integrated Field Research site [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 613 Personal health and safety
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
• Mô tả vật lý: Size: Article No. e0137270 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261100
• Chủ đề: 1. 12 management of radioactive and non-radioactive wastes from nuclear facilities; 2. 60 applied life sciences; 3. Bacteria; 4. Bromides; 5. Reverse transcriptase-polymerase chain reaction; 6. Ribosomal rna; 7. Ribosomes; 8. Sulfates; 9. Sulfides; 10. Uranium

Tóm tắt

 Understanding which organisms are capable of reducing uranium at historically contaminated sites provides crucial information needed to evaluate treatment options and outcomes. One approach is determination of the bacteria which directly respond to uranium addition. In this research, uranium amendments were made to groundwater samples from a site of ongoing biostimulation with acetate. The active microbes in the planktonic phase were deduced by monitoring ribosomes production via RT-PCR. The results indicated several microorganisms were synthesizing ribosomes in proportion with uranium amendment up to 2 ?M. Concentrations of U (VI) >
 2 ?M were generally found to inhibit ribosome synthesis. Two active bacteria responding to uranium addition in the field were close relatives of <
 i>
 Desulfobacter postgateii<
 /i>
  and <
 i>
 Geobacter bemidjiensis<
 /i>
 . Since RNA content often increases with growth rate, our findings suggest it is possible to rapidly elucidate active bacteria responding to the addition of uranium in field samples and provides a more targeted approach to stimulate specific populations to enhance radionuclide reduction in contaminated sites.