Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 539.7 Atomic and nuclear physics
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
• Mô tả vật lý: Size: Article No. e0117437 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261225
• Chủ đề: 1. 46 instrumentation related to nuclear science and technology; 2. 59 basic biological sciences; 3. Dehydration (medicine); 4. Et al; 5. Fibroblasts; 6. Fluorescence imaging; 7. Fluorescence microscopy; 8. Liquid nitrogen; 9. Ultraviolet radiation; 10. X-ray microscopy; 11. X-ray radiography

Tóm tắt

Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with x-ray fluorescence microscopy. We show that the contents and distributions of most biologically important elements remain nearly unchanged when compared with non-ultraviolet-irradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results.