Subcellular analysis by laser ablation electrospray ionization mass spectrometry [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 617 Miscellaneous branches of medicine Surgery
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2014
• Mô tả vật lý: Medium: ED : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261386
• Chủ đề: 1. 47 other instrumentation; 2. 59 basic biological sciences; 3. 60 applied life sciences

Tóm tắt

In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.