Efficient Cisplatin Pro-Drug Delivery Visualized with Sub-100 nm Resolution [electronic resource] : Interfacing Engineered Thermosensitive Magnetomicelles with a Living System

• Ngôn ngữ: eng
• Ký hiệu phân loại: 612 Human physiology
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2014
• Mô tả vật lý: Size: Article No. 1400182 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261400
• Chủ đề: 1. 60 applied life sciences; 2. Drug delivery; 3. Hard x-ray fluorescence nanoprobe; 4. Nano-bio interfaces; 5. Sub-100 nm resolution; 6. Thermosensitive magnetomicelles

Tóm tắt

 Temperature-responsive magnetic nanomicelles can serve as thermal energy and cargo carriers with controlled drug release functionality. In view of their potential biomedical applications, understanding the modes of interaction between nanomaterials and living systems and evaluation of efficiency of cargo delivery is of the utmost importance. In this paper, we investigate the interaction between the hybrid magnetic nanomicelles engineered for controlled platinum complex drug delivery and a biological system at three fundamental levels: subcellular compartments, a single cell and whole living animal. Nanomicelles with polymeric P(NIPAAm-co-AAm)-b-PCL core-shell were loaded with a hydrophobic Pt(IV) complex and Fe<
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  nanoparticles though self-assembly. The distribution of a platinum complex on subcellular level is visualized using hard X-ray fluorescence microscopy with unprecedented level of detail at sub-100 nm spatial resolution. We then study the cytotoxic effects of platinum complex-loaded micelles in vitro on a head and neck cancer cell culture model SQ20B. In conclusion, by employing the magnetic functionality of the micelles and additionally loading them with a near infrared fluorescent dye, we magnetically target them to a tumor site in a live animal xenografted model which allows to visualize their biodistribution in vivo.