Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 545.3 Quantitative analysis
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2014
• Mô tả vật lý: Medium: ED : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261442
• Chủ đề: 1. 60 applied life sciences

Tóm tắt

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.