Antibody profiling sensitivity through increased reporter antibody layering [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 571.6 Cell biology

Thông tin xuất bản: Idaho Falls, Idaho : Oak Ridge, Tenn. : Idaho National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2013

Mô tả vật lý: Medium: ED : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 261542

 A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known
  contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes
  washing away antibodies that do form immune complexes
  and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.
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