Methods of increasing secretion of polypeptides having biological activity [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 636.7 Dogs

Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2013

Mô tả vật lý: Medium: ED : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 261558

 The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide
  (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof
  and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity
  wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase
  (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein
  and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.
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