The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide
(ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof
and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity
wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase
(b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein
and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.