Compositions and methods for RNA-based antimicrobial drug targeting [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 541 Physical chemistry

Thông tin xuất bản: Los Alamos, N.M. : Oak Ridge, Tenn. : Los Alamos National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2019

Mô tả vật lý: Medium: ED : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 261845

A ribonucleic acid (RNA) based modular regulatory element (MRE) for inhibiting translation of a target gene transcript in an organism (e.g. a pathogen) includes a first segment of RNA capable of forming a stem loop structure, a second segment of RNA downstream of the first segment of RNA and having a target sequence capable of binding to the target gene transcript (or polycistron lead gene transcript), and a third segment of RNA downstream of the second segment of RNA and being capable of forming a terminator hairpin downstream of the target sequence. Methods for assaying the role of a putative target gene in an organism include inhibiting expression of various target genes using the RNA-based MRE.
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