From the Soil to the Seed. Metal Transport in Arabidopsis [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 631.5 Cultivation and harvesting
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
• Mô tả vật lý: Size: 10 p. : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262109
• Chủ đề: 1. 37 inorganic, organic, physical, and analytical chemistry; 2. 59 basic biological sciences; 3. 60 applied life sciences; 4. 71 classical and quantum mechanics, general physics; 5. Arabidopsis, synchrotron x-ray fluorescence analysis, metals, iron, manganese, zinc, metal transport

Tóm tắt

Deficiencies of micronutrients such as Fe, Mn, and Zn commonly limit plant growth and crop yields. The long-term goals of our program are to understand how plants acquire metal micronutrients from the soil and distribute them while protecting themselves from the potential redox damage metals can cause to living tissues. Metals serve as important co-factors for photosynthesis and respiration, yet we still know very little about metal transport. Our approach combines experimental and computational tools from the physical sciences with biochemistry and molecular biology. Specifically, we combine mutant analysis with synchrotron X-ray fluorescence (SXRF) spectroscopy, a technique that allows us to image the elemental composition of living plant material in 3-D. By analyzing the phenotypes of lines carrying mutations in various metal transporters, we have identified the genes responsible for uptake of zinc from the soil as well as genes involved in loading the seeds with metal micronutrients. Several of these transporters affect the localization of metals in the seed without affecting the overall metal content. Understanding how seeds obtain and store nutrients is key to developing crops with higher agronomic and nutritional value.