Next generation sequencing (NGS)technologies and applications [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 576.5 Genetics
• Thông tin xuất bản: Los Alamos, N.M. : Oak Ridge, Tenn. : Los Alamos National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2012
• Mô tả vật lý: Medium: ED : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262230
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Amplification; 4. Data analysis; 5. Genes; 6. Nucleic acids; 7. Quality control; 8. Rna; 9. Synthesis

Tóm tắt

 NGS technology overview: (1) NGS library preparation - Nucleic acids extraction, Sample quality control, RNA conversion to cDNA, Addition of sequencing adapters, Quality control of library
  (2) Sequencing - Clonal amplification of library fragments, (except PacBio), Sequencing by synthesis, Data output (reads and quality)
  and (3) Data analysis - Read mapping, Genome assembly, Gene expression, Operon structure, sRNA discovery, and Epigenetic analyses.