Interim report on updated microarray probes for the LLNL Burkholderia pseudomallei SNP array [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 577.2 Specific factors affecting ecology
• Thông tin xuất bản: Livermore, Calif : Oak Ridge, Tenn. : Lawrence Livermore National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2012
• Mô tả vật lý: Size: PDF-file: 9 pages
• Bộ sưu tập: Metadata
• ID: 262269
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Design; 4. Genetics; 5. Lawrence livermore national laboratory; 6. Nucleotides; 7. Probes

Tóm tắt

The overall goal of this project is to forensically characterize 100 unknown Burkholderia isolates in the US-Australia collaboration. We will identify genome-wide single nucleotide polymorphisms (SNPs) from B. pseudomallei and near neighbor species including B. mallei, B. thailandensis and B. oklahomensis. We will design microarray probes to detect these SNP markers and analyze 100 Burkholderia genomic DNAs extracted from environmental, clinical and near neighbor isolates from Australian collaborators on the Burkholderia SNP microarray. We will analyze the microarray genotyping results to characterize the genetic diversity of these new isolates and triage the samples for whole genome sequencing. In this interim report, we described the SNP analysis and the microarray probe design for the Burkholderia SNP microarray.