Deciphering the details of RNA aminoglycoside interactions [electronic resource] : from atomistic models to biotechnological applications

• Ngôn ngữ: eng
• Ký hiệu phân loại: 576.8 Evolution
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2012
• Mô tả vật lý: Size: 222 p. : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262291
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences

Tóm tắt

A detailed study was done of the neomycin-B RNA aptamer for determining its selectivity and binding ability to both neomycin? and kanamycin-class aminoglycosides. A novel method to increase drug concentrations in cells for more efficiently killing is described. To test the method, a bacterial model system was adopted and several small RNA molecules interacting with aminoglycosides were cloned downstream of T7 RNA polymerase promoter in an expression vector. Then, the growth analysis of E. coli expressing aptamers was observed for 12-hour period. Our analysis indicated that aptamers helped to increase the intracellular concentration of aminoglycosides thereby increasing their efficacy.