Reenacting the birth of an intron [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 571.6 Cell biology
• Thông tin xuất bản: Berkeley, Calif. : Oak Ridge, Tenn. : Lawrence Berkeley National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2011
• Mô tả vật lý: Medium: ED : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262342
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Functionals; 4. In vivo; 5. Introns; 6. Nucleotides; 7. Splicing; 8. Vertebrates

Tóm tắt

An intron is an extended genomic feature whose function requires multiple constrained positions - donor and acceptor splice sites, a branch point, a polypyrimidine tract and suitable splicing enhancers - that may be distributed over hundreds or thousands of nucleotides. New introns are therefore unlikely to emerge by incremental accumulation of functional sub-elements. Here we demonstrate that a functional intron can be created de novo in a single step by a segmental genomic duplication. This experiment recapitulates in vivo the birth of an intron that arose in the ancestral jawed vertebrate lineage nearly half a billion years ago.