ChIP-seq Mapping of Distant-Acting Enhancers and Their In Vivo Activities [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 576.8 Evolution
• Thông tin xuất bản: Berkeley, Calif. : Oak Ridge, Tenn. : Lawrence Berkeley National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2011
• Mô tả vật lý: Size: 1 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262350
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. 99 general and miscellaneousmathematics, computing, and information science; 4. Genes; 5. In vivo; 6. Mouse, genomic location, chip-seq mapping, distant-acting enhancers; 7. Proteins

Tóm tắt

The genomic location and function of most distant-acting transcriptional enhancers in the human genome remains unknown We performed ChIP-seq for various transcriptional coactivator proteins (such as p300) directly from different embryonic mouse tissues, identifying thousands of binding sitesTransgenic mouse experiments show that p300 and other co-activator peaks are highly predictive of genomic location AND tissue-specific activity patterns of distant-acting enhancersMost enhancers are active only in one or very few tissues Genomic location of tissue-specific p300 peaks correlates with tissue-specific expression of nearby genes Most binding sites are conserved, but the global degree of conservation varies between tissues