Antibody binding to p-Si using LANL SAM chemistry [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 504.7 [Unassigned]
• Thông tin xuất bản: Los Alamos, N.M. : Oak Ridge, Tenn. : Los Alamos National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2010
• Mô tả vật lý: Medium: ED : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262379
• Chủ đề: 1. 60 applied life sciences; 2. Antibodies; 3. Coatings; 4. Lanl; 5. Meetings; 6. Silicon; 7. Surfaces

Tóm tắt

This NMSBA-sponsored project involves the attachment of antibodies to polymeric silicon (p-Si) surfaces, with the ultimate goal of attaching antibodies to nanowires for Vista Therapeutics, Inc. (Santa Fe, NM). This presentation describes the functionalization of p-Si surfaces. the activation of terminal carboxylates on these surfaces, the conjugation of antibodies, and the analyses undertaken at each step. The results of this work show that antibody conjugation is possible on p-Si coatings using the well-known EDC/NHS activation chemistry.