A bio-synthetic interface for discovery of viral entry mechanisms. [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 574,87 [Unassigned]
• Thông tin xuất bản: Albuquerque, N.M. : Oak Ridge, Tenn. : Sandia National Laboratories. ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2010
• Mô tả vật lý: Size: 23 p. : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262408
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Interfaces; 4. Lipids; 5. Membrane transport; 6. Viruses

Tóm tắt

Understanding and defending against pathogenic viruses is an important public health and biodefense challenge. The focus of our LDRD project has been to uncover the mechanisms enveloped viruses use to identify and invade host cells. We have constructed interfaces between viral particles and synthetic lipid bilayers. This approach provides a minimal setting for investigating the initial events of host-virus interaction - (i) recognition of, and (ii) entry into the host via membrane fusion. This understanding could enable rational design of therapeutics that block viral entry as well as future construction of synthetic, non-proliferating sensors that detect live virus in the environment. We have observed fusion between synthetic lipid vesicles and Vesicular Stomatitis virus particles, and we have observed interactions between Nipah virus-like particles and supported lipid bilayers and giant unilamellar vesicles.