Establishing <em>Butyribacterium methylotrophicum</em> as a Platform Organism for the Production of Biocommodities from Liquid C<sub>1</sub> Metabolites [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 576.5 Genetics

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2022

Mô tả vật lý: Size: Article No. e02393-21 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 262464

 Using the Wood-Ljungdahl pathway, acetogens can nonphotosynthetically fix gaseous C<
 sub>
 1<
 /sub>
  molecules, preventing them from entering the atmosphere. Many acetogens can also grow on liquid C<
 sub>
 1<
 /sub>
  compounds such as formate and methanol, which avoid the storage and mass transfer issues associated with gaseous C<
 sub>
 1<
 /sub>
  compounds. Substrate redox state also plays an important role in acetogen metabolism and can modulate products formed by these organisms. Butyribacterium methylotrophicum is an acetogen known for its ability to synthesize longer-chained molecules such as butyrate and butanol, which have significantly higher values than acetate or ethanol, from one-carbon (C<
 sub>
 1<
 /sub>
 ) compounds. We explored B. methylotrophicum?s C<
 sub>
 1<
 /sub>
  metabolism by varying substrates, substrate concentrations, and substrate feeding strategies to improve four-carbon product titers. Our results showed that formate utilization by B. methylotrophicum favored acetate production and methanol utilization favored butyrate production. Cofeeding of both substrates produced a high butyrate titer of 4 g/liter when methanol was supplied in excess to formate. Testing of formate feeding strategies, in the presence of methanol, led to further increases in the butyrate to acetate ratio. Mixotrophic growth of liquid and gaseous C<
 sub>
 1<
 /sub>
  substrates expanded the B. methylotrophicum product profile, as ethanol, butanol, and lactate were produced under these conditions. We also showed that B. methylotrophicum is capable of producing caproate, a six-carbon product, presumably through chain elongation cycles of the reverse ?-oxidation pathway. Furthermore, we demonstrated butanol production via heterologous gene expression. Finally, our results indicate that both selection of appropriate substrates and genetic engineering play important roles in determining titers of desired products.
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