A multi-omic characterization of temperature stress in a halotolerant <em>Scenedesmus</em> strain for algal biotechnology [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 577.8 Synecology and population biology
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2021
• Mô tả vật lý: Size: 2.4 MB : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262532
• Chủ đề: 1. 09 biomass fuels; 2. Biofuel algae; 3. Chlorophyceae; 4. Genome; 5. Scenedesmaceae

Tóm tắt

 Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 �C that reaches 30 g/m<
 sup>
 2<
 /sup>
 /day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.