Visualizing plant cell wall changes proves the superiority of hydrochloric acid over sulfuric acid catalyzed ?-valerolactone pretreatment [electronic resource]

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Ngôn ngữ: eng

Ký hiệu phân loại: 666.3 Pottery

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2021

Mô tả vật lý: Size: Article no. 128660 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 262571

 Thermochemical pretreatment is one of the key steps to process lignocellulosic biomass for production of biofuels and biomaterials. ?-valerolactone (GVL) has been used as a green and renewable solvent to efficiently dissolve lignin under acidic conditions and enhance subsequent enzyme digestibility. Further improvement is still required to lower the pretreatment temperature in order to reduce sugar degradation and irreversible lignin condensation, as well as the capital cost. In this study, we compared the use of HCl and H<
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 SO<
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  as catalysts during GVL pretreatment, and found the performance of GVL-HCl at 100 �C was comparable to that of GVL-H<
 sub>
 2<
 /sub>
 SO<
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 4<
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  at 120 �C in terms of xylan and lignin removal and enzyme digestibility. We further monitored the lignin removal and cellulose accessibility in the cell walls at different pretreatment time points by imaging the changes in lignin auto-fluorescence and in CtCBM3-GFP binding with confocal laser scanning microscopy (CLSM), respectively. We found GVL-HCl pretreatment at a relative low temperature (100 �C) could rapidly remove lignin in the compound middle lamella and cell corner areas, which concurred with the increase of CtCBM3-GFP binding in these areas. Real-time imaging of cell wall degradation by cellulases further revealed that the secondary cell walls could be digested from both cell lumen and CML sides, and eventually fully deconstructed within 24 h. Furthermore, our results provide new insight into the effects of chloride anions on plant cell wall structure during GVL pretreatment, and offer potential routes to further optimize and enhance the efficiency of GVL-based pretreatment.
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