Adaptive Laboratory Evolution for algal strain improvement [electronic resource] : methodologies and applications

• Ngôn ngữ: eng
• Ký hiệu phân loại: 668.4 Plastics
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2020
• Mô tả vật lý: Size: Article No. 102122 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262580
• Chủ đề: 1. 09 biomass fuels; 2. 59 basic biological sciences; 3. Adaptive laboratory evolution (ale); 4. Biotechnology & applied microbiology; 5. Strain improvement

Tóm tắt

Originally developed in bacterial and fungal models, Adaptive Laboratory Evolution (ALE) experimentally selects for targeted phenotypes by allowing cells containing beneficial random genetic mutations to replicate more quickly in the presence of environmental stress. When applied to microalgae, this technique has been used to increase growth rate, stress tolerance, substrate utilization, and product yield. This review discusses ALE experimental design parameters, and summarizes key results and lessons learned from recent microalgal ALE studies. By documenting the current state of the field, we provide insight on the ways microalgal ALE might be used to develop commercially viable biotechnology strains.