Valorization of CO<sub>2</sub> through lithoautotrophic production of sustainable chemicals in <em>Cupriavidus necator</em> [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 666.3 Pottery

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2020

Mô tả vật lý: Size: p. 207-220 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 262612

 Coupling recent advancements in genetic engineering of diverse microbes and gas-driven fermentation provides a path toward sustainable commodity chemical production. Cupriavidus necator H16 is a suitable species for this task because it effectively utilizes H<
 sub>
 2<
 /sub>
  and CO<
 sub>
 2<
 /sub>
  and is genetically tractable. Here, we demonstrate the versatility of C. necator for chemical production by engineering it to produce three products from CO<
 sub>
 2<
 /sub>
  under lithotrophic conditions: sucrose, polyhydroxyalkanoates (PHAs), and lipochitooligosaccharides (LCOs). We engineered sucrose production in a co-culture system with heterotrophic growth 30 times that of WT C. necator. We engineered PHA production (20-60% DCW) and selectively altered product composition by combining different thioesterases and phaCs to produce copolymers directly from CO<
 sub>
 2<
 /sub>
 . And, we engineered C. necator to convert CO<
 sub>
 2<
 /sub>
  into the LCO, a plant growth enhancer, with titers of ~1.4 mg/L?equivalent to yields in its native source, Bradyrhizobium. Here, we applied the LCOs to germinating seeds as well as corn plants and observed increases in a variety of growth parameters. Taken together, these results expand our understanding of how a gas-utilizing bacteria can promote sustainable production.
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