Improving biomass and lipid yields of <em>Desmodesmus armatus</em> and <em>Chlorella vulgaris</em> through mutagenesis and high-throughput screening [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 666.9 Masonry adhesives
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2020
• Mô tả vật lý: Size: Article No. 105755 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262614
• Chủ đề: 1. 09 biomass fuels; 2. 59 basic biological sciences; 3. 60 applied life sciences; 4. Microalgae; 5. Mutagenesis

Tóm tắt

Advancement of microalgae for use as biofuel feedstock depends on bioprospecting novel strains and then applying classical strain improvement techniques to enhance desirable traits. Here we successfully demonstrate this approach using two novel strains of green algae, identified as Desmodesmus armatus and Chlorella vulgaris, to improve biomass and lipid yields. UV irradiation was utilized to generate random mutations and the resulting populations were analyzed via flow cytometry to quantify lipid fluorescence while enriching for high lipid variants using fluorescence activated cell sorting. Secondary screening was performed via in situ fluorescence staining to identify the single highest lipid producing mutants from each strain. This approach produced a Desmodesmus armatus mutant that exhibited both increased biomass productivity and higher lipid content, which doubled lipid yield from the culture. The resulting Chlorella vulgaris mutant exhibited an increase in growth only, resulting in a 33% increase in lipid yield.