Metabolic engineering of <em>Escherichia coli</em> for the production of butyric acid at high titer and productivity [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 660.6 Biotechnology
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Policy and International Affairs ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2019
• Mô tả vật lý: Size: Article No. 62 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262838
• Chủ đề: 1. 09 biomass fuels; 2. 59 basic biological sciences; 3. Biotechnology & applied microbiology; 4. Butyrate; 5. Energy & fuels

Tóm tắt

Background: Several anaerobic bacteria produce butyric acid, a commodity chemical with use in chemical, pharmaceutical, food and feed industries, using complex media with acetate as a co- product. Butyrate titer of various recombinant Escherichia coli did not exceed 10 g.l-1 in batch fermentations in any of the media tested. Results: A recombinant E. coli (strain LW393) that produced butyrate as the major fermentation product was constructed with genes from E. coli, Clostridium acetobutylicum and Treponema denticola. Strain LW393 produced 323 � 6 mM (28.4 � 0.4 g.l-1) butyric acid in batch fermentations in mineral salts medium with glucose as C-source at a yield of 0.37 � 0.01 g.(g -1 glucose consumed) Supplementing this medium with yeast extract further increased butyric acid titer to 375 � 4 mM. . Butyrate accounted for 90% of the total products produced by the culture. -1 -1 Average volumetric productivity of butyrate with xylose as C-source was 0.89 � 0.07 g.l .h . Conclusions: The butyrate titer reported in this study is about 2.5-3-times higher than the values reported for other recombinant E. coli and this is achieved in mineral salts medium with an expectation of lower purification and production cost of butyrate.