Metabolic engineering of <em>Clostridium carboxidivorans</em> for enhanced ethanol and butanol production from syngas and glucose [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 660.6 Biotechnology
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2019
• Mô tả vật lý: Size: p. 415-423 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262840
• Chủ đề: 1. 09 biomass fuels; 2. Aldehyde:ferredoxin oxidoreductase; 3. Aldehydealcohol dehydrogenase; 4. Clostridium carboxidivorans; 5. Ferredoxin-nad+ reductase

Tóm tắt

 <
 em>
 Clostridium carboxidivorans<
 /em>
  can convert CO<
 sub>
 2<
 /sub>
 , CO and H<
 sub>
 2<
 /sub>
  to ethanol and <
 em>
 n<
 /em>
 -butanol
  however, its industrial application is limited by the lack of tools for metabolic pathway engineering. In this study, <
 em>
 C. carboxidivorans<
 /em>
  was successfully engineered to overexpress <
 em>
 aor<
 /em>
 , <
 em>
 adhE2<
 /em>
 , and <
 em>
 fnr<
 /em>
  together with <
 em>
 adhE2<
 /em>
  or <
 em>
 aor<
 /em>
 . In glucose fermentation, all engineered strains showed higher alcohol yields compared to the wild-type. Strains overexpressing aor showed CO<
 sub>
 2<
 /sub>
  re-assimilation during heterotrophic growth. In syngas fermentation, compared to the wild-type, the strain overexpressing <
 em>
 adhE2<
 /em>
  produced ~50% more ethanol and the strain overexpressing <
 em>
 adhE2<
 /em>
  and <
 em>
 fnr<
 /em>
  produced ~18% more butanol and ~22% more ethanol. Interestingly, both strains showed obvious acid re-assimilation, with <
 0.15 g/L total acid remaining at the end of fermentation. Overexpressing <
 em>
 fnr<
 /em>
  with <
 em>
 adhE2<
 /em>
  enhanced butanol production compared to only <
 em>
 adhE2<
 /em>
 . Furthermore, this is the first report of overexpressing homologous and heterologous genes in <
 em>
 C. carboxidivorans<
 /em>
  for enhancing alcohols production from syngas and glucose.