Direct conversion of cellulose into ethanol and ethyl-?-<scp>D</scp>-glucoside via engineered <em>Saccharomyces cerevisiae</em> [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 621.48 Nuclear engineering
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2018
• Mô tả vật lý: Size: p. 2859-2868 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262961
• Chủ đề: 1. 09 biomass fuels; 2. Cellulose; 3. Simultaneous saccharification and fermentation; 4. Transglycosylation; 5. Yeast

Tóm tắt

 Simultaneous saccharification and fermentation (SSF) of cellulose via engineered <
 em>
 Saccharomyces cerevisiae<
 /em>
  is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio?degradable surfactant, ethyl???d?glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT?1) and intracellular ??glucosidase (GH1?1) originating from <
 em>
 Neurospora crassa<
 /em>
 . We identified the formation of ethyl???d?glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1?1. The EJ2 strain coproduced 0.34 � 0.03 g ethanol/g cellulose and 0.06 � 0.00 g ethyl???d?glucoside/g cellulose at a rate of 0.30 � 0.02 g/L/h and 0.09 � 01 g?L<
 sup>
 -1<
 /sup>
 ?h<
 sup>
 -1<
 /sup>
 , respectively, during the SSF of Avicel PH?101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co?production of a value?added chemical (alkyl?glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1?1 in engineered <
 em>
 S. cerevisiae<
 /em>
 . In conclusion, this co?production could have a substantial effect on overall techno?economic feasibility of SSF of cellulose.