In vivo synergistic activity of a CAZyme cassette from <i>Acidothermus cellulolyticus</i> significantly improves the cellulolytic activity of the <i>C. bescii</i> exoproteome [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 547.2 Organic chemical reactions formerly 547.139

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017

Mô tả vật lý: Size: p. 2474-2480 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263223

 The use of microbial cells to convert plant biomass directly to fuels and chemicals is referred to as consolidated bioprocessing (CBP). Members of the bacterial genus, <
 i>
 Caldicellulosiruptor<
 /i>
  (Gram-positive, anaerobic hyperthermophiles) are capable of deconstructing plant biomass without enzymatic or chemical pretreatment. This is accomplished by the production and secretion of free, multi-domain enzymes that outperform commercial enzyme cocktails on some substrates. Here, we show that the exoproteome of <
 i>
 C. bescii<
 /i>
  may be enhanced by the heterologous expression of enzymes from Acidothermus cellulolyticus that act synergistically to improve sugar release from complex substrates
  as well as improve cell growth. In this work, co-expression of the <
 i>
 A. cellulolyticus<
 /i>
  Acel_0615 ..beta..-glucanase (GH6 and GH12) and E1 endoglucanase (GH5) enzymes resulted in an increase in the activity of the exoproteome on Avicel
  as well as an increase in growth of <
 i>
 C. bescii<
 /i>
  on Avicel compared to the parental strain or the strain expressing the ..beta..-glucanase alone. As a result, our ability to engineer the composition and effectiveness of the exoproteome of these bacteria provides insight into the natural mechanism of plant cell wall deconstruction, as well as future directions for improving CBP.
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